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KMID : 0364820120480020141
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Korean Journal of Microbiology
2012 Volume.48 No. 2 p.141 ~ p.146
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Cloning and Characterization of Xylanase Gene from Paenibacillus woosongensis
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Yoon Ki-Hong
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Abstract
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A gene encoding the xylanase (XynA) predicted from partial genomic sequence of Paenibacillus woosongensis was
cloned into Escherichia coli by PCR. This xynA gene consisted of 633 nucleotides, encoding a polypeptide of 211
amino acid residues. The deduced amino acid sequence exhibited 85-89% identity with those of several
Paenibacillus xylanases, belonging to the glycosyl hydrolase family 11. As a results of expression of the structural
gene by T7 promoter of a pET23a(+) expression vector, xylanase activity was higher in cell-free extract than culture
filtrate of a recombinant Escherichia coli BL21(DE3) CodonPlus. However, the expression level of xylanase was not
sufficient be detected by SDS-PAGE. The cell-free extract showed maximal xylanase activity at 60℃and pH 5.5.
The predominant products resulting from xylan and xylooligosaccharide hydrolysis were xylose and xylotriose. The
enzyme could hydrolyze xylooligosaccharides larger than xylbiose.
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KEYWORD
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Paenibacillus woosongensis, cloning, reaction property, xylanase
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